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Name of the ingredient
Hemp seed oil (AAN# 144625)
Definition of the ingredient
Hemp seed oil is a fatty oil that is obtained from the cold-pressing of de-hulled Cannabis sativa seeds. Hemp seed oil may only contain trace amounts of cannabidiol (CBD) limited to ≤75 ppm and/or tetrahydrocannabinols (THC) limited to ≤10 ppm.
CAS Number: 8016-24-8
| Test | Method reference | Acceptance criteria |
|---|---|---|
| Description | ||
|
Appearance/colour |
Visual |
Yellow |
|
Flavour/odour |
Organoleptic |
Slightly Nutty |
|
Lovibond colour |
ISO 15305:1998 |
Yellow: ≤35 Red: ≤3.0 |
| Characteristics | ||
|
Moisture |
ISO 662:2016 |
≤0.15% |
|
Insoluble impurities content |
ISO 663:2017 |
≤0.05% |
|
Peroxide value |
ISO 3960:2017 |
≤8 meq/Kg |
|
Acid value |
ISO 660:2020 |
≤3 mg/g |
| Identification | ||
|
Chemical 'fingerprint' |
GC-MS[1] |
The GC-MS scan of the hemp seed oil uniquely identifies the presence of residual cannabinoids D9-THC (21.87 min) and CBD (21.09 min) by matching both retention time and Mass Spectral data of the unknown and standards to a searchable Mass spectral database traceable to NIST from which one can uniquely identify the hemp seed oil from other edible oils. |
|
Chemical 'fingerprint' |
HPLC-UV[2] |
The UPLC-UV chromatograms of hemp seed oil uniquely identifies the presence of four residual cannabinoids, namely D9-THC (5.67 min) and CBD (3.24 min). |
| Assay | ||
|
Cannabidiol |
HPLC-MS[2, 3] |
≤75 mg/Kg |
|
Tetrahydrocannabinols |
HPLC-MS[2, 3] |
≤10 mg/Kg |
|
Incidental metals and non-metals |
||
|---|---|---|
|
Lead |
BS EN ISO 17294-2:2016 |
≤0.1 mg/Kg |
|
Arsenic |
BS EN ISO 17294-2:2016 |
≤0.1 mg/Kg |
|
Mercury |
BS EN ISO 17294-2:2016 |
≤0.1 mg/Kg |
|
Cadmium |
BS EN ISO 17294-2:2016 |
≤0.1 mg/Kg |
|
Pesticide residues and environmental contaminants: (including agricultural and veterinary substances) |
||
|
Pesticide residues |
BS EN 12393:2013 |
Not detected |
|
Other organic or inorganic impurities or toxins |
||
|
Aflatoxin B1 |
DIN EN ISO 14123:2008 |
≤ 2.0 ppb |
|
Aflatoxin B1+B2+G1+G2 |
DIN EN ISO 14123:2008 |
≤4.0 ppb |
| Microbiology | ||
|
While substance manufacturers are encouraged to include limits for objectionable microorganisms, it is the product into which those substances are formulated that is subject to a legally binding set of criteria. The Therapeutic Goods Order No. 100 'Microbiological Standards for Medicines' mandates that any finished product that contains the ingredient, alone or in combination with other ingredients, must comply with the microbial acceptance criteria set by Clause 11 of the Order. |
||
Footnotes
| [1] |
GC-MS: Column: HP-5MS Ultra inert 30 m, 0.25 mm ID, 0.25 µm film or equivalent; Scan Mode: 35-450 amu; Injection Mode: Splitless; Injection volume: 1 mL; Injector Temperature: 280oC; Source Temperature: 230oC; Flow Rate: 1 mL/min He; Oven initial: 75°C → Hold 2 min → 10°C/min to Oven Final: 300°C → Hold 5 min; MS Transfer Line: 280°C; Scan: 3 cycles/sec, 334.8 ms/cycle; EMV: 1730 eV. |
|---|---|
| [2] |
HPLC-UV: Column: Acquity UPLC BEH C18, 50 x 2.1 mm, 1.7 μm; Mobile phase A: 100 mM Formic acid; Mobile Phase B: ACN; Mobile phase composition: A: 20% & B: 80%; Flow rate: 0.5 mL/min; Column temperature: 40°C; Wavelength: 229 nm. |
| [3] | HPLC-MS: Column: Acquity UPLC BEH C18, 50 x 2.1 mm, 1.7 μm; Mobile phase A: 100 mM Formic acid; Mobile Phase B: ACN; Mobile phase composition: A: 20% & B: 80%; Flow rate: 0.5 mL/min; Column temperature: 40°C; MS ionisation mode: ESI; MS Source Temp: 120°C; MS Desolvation Temp: 600°C; MS Cone Gas Flow: 50 L/Hr; MS De-solvation Gas Flow: 1000 L/Hr. |
Key to Abbreviations:
BS = British Standards Institute
CBD = Cannabidiol
D9-THC = delta-9-tetrahydrocannabinol
DIN = Deutsches Institut fur Normung (German Institute for Standardisation)
EN = English
GC = Gas Chromatography
HPLC = High performance liquid chromatography
ISO = International Organisation for Standardisation
MS = Mass Spectrometry
NIST = National Institute of Standards and Technology
UV = Ultraviolet